A researcher studying the enzyme lactase notes that the enzyme's activity is most optimal at a pH of 6.8. If the pH of the reaction mixture is increased to 7.4, what will happen to the enzyme's activity?

Core Concept

This question requires understanding of enzyme kinetics, specifically the relationship between pH and enzyme activity. Most enzymes, including lactase, have an optimal pH range within which they are most active. Increasing the pH of the reaction mixture beyond the optimal range can denature the enzyme, leading to a decrease in its activity. This is due to the change in the electrostatic interactions between the enzyme and its substrate, making it harder for the enzyme to bind and catalyze the reaction.